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. 2019 Jul 9;9(17):5105–5121. doi: 10.7150/thno.33904

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Proliferation, apoptosis, and viability of CD146⁺ ADSCs before and after MACS enrichment. (A) Morphology of CD146⁺ ADSCs and donor-matched ADSCs on day 1, 3, and 5. Cells were cultured at the same density on day 1. Scale bar: 50 μm. (B) Cell proliferation CCK8 assay of ADSCs and CD146⁺ ADSCs. *P < 0.05, pairwise comparisons, Student's t-test. (C) Apoptosis in ADSCs and CD146⁺ subpopulation was determined by flow cytometric analysis. (D) The proportion of apoptotic ADSCs and CD146⁺ subpopulation. The proportions of live, early apoptotic, late apoptotic, debris, and dead cells of the CD146⁺ subpopulation are shown in mean ± standard error, ***P < 0.05 by Student's t-test compared with ADSCs. (E) Viability in ADSCs and CD146⁺ cells by flow cytometry. (F) Cell viability of ADSCs and CD146⁺ subpopulation. The proportions of live and dead CD146⁺ cells are shown in the mean ± standard error of the mean, ***P < 0.05 by Student's t-test compared with ADSCs.