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. 2019 Jul 9;9(17):5105–5121. doi: 10.7150/thno.33904

Figure 6.

Figure 6

Fabrication and biocompatibility of cell-scaffold composites. (A) Experimental design of the cell-scaffold composites. ADSCs and CD146+ cells were seeded into an ACECM scaffold for 3 days. (B) Macroscopic features of the ACECM scaffold and two cell-scaffold composites. (C) SEM images of the ACECM scaffold and two cell-scaffold composites. (D) Live/dead cell analysis for the two cell-scaffold composites. Representative images show dead cells (red), live cells (green), and reconstruction images of the two cell-scaffold composites. (E) Photographs of the ACECM scaffold and two cell-scaffold composites subcutaneously implanted in the back of rabbits after 1 week, 2 weeks, and 4 weeks (the circle indicates the tissue surrounding the scaffold). (F) Immunofluorescent staining of HLA-ABC of the tissues surrounding the implant sites after 1 week, 2 weeks, and 4 weeks. Scale bar: 50 μm. (G) H&E staining of the tissues surrounding the implant sites after 1 week, 2 weeks, and 4 weeks. Scale bar: 100 μm.