Skip to main content
. 2019 Jul 9;9(17):4878–4892. doi: 10.7150/thno.36254

Figure 2.

Figure 2

Brigatinib induces apoptosis by activating ER stress in CRC cells. A, Immunoblotting of total and phosphorylated PERK, IRE1α and CHOP in CRC cells treated with the indicated concentrations of brigatinib for 24 hours. B, Interaction among BiP, PERK, and IRE1α was determined by coimmunoprecipitation assay. C, Immunoblotting of total and phosphorylated PERK, IRE1α and CHOP as well as total and cleaved PARP or caspase 3 in CRC cells treated with or without 1 μM brigatinib in the presence or absence of 2 mM 4-phenylbutyrate (4-PBA) for 24 hours. D, Colony formation assay of CRC cells treated with or without 1 μM brigatinib in the presence or absence of 2 mM 4-PBA. Representative images (Top) and Quantification of colonies (Bottom) were shown. **, P < 0.01. E-G, Cell growth assay (E), LDH release assay (F) and flow cytometric analysis of apoptosis (G) in CRC cells treated as (D). *, P < 0.05; **, P < 0.01.