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. 2019 Jul 9;9(17):4909–4922. doi: 10.7150/thno.33758

Figure 1.

Figure 1

SMAC-mimetics induce survivin-dependent mitochondrial re-organisation. SH-EP/Ctr (A), SH-EP/Surv (B) or SH-EP/shSurv (C) cells were seeded on glass slides and treated for 24 hours with either 10 µM LCL161 or 10 µM TL32711. Mitochondria were stained with 300 nM CMXRos and analyzed with a 63x oil objective in an Axiovert200M microscope equipped with an ApoTome.2 system. Shown are representative images. Bar is 10 µm. (D) For quantification, at least 60 cells from three independent experiments were analyzed for mitochondrial morphology. ***P<0.001. (E) Mitochondrial staining of SH-EP/shCtr and SH-EP/shXIAP cells (CMXRos 300 nM). Bar is 10 µm. For quantification at least 90 cells out of four independent experiments were analyzed for tubular or fragmented mitochondria. ***P<0.001.