Figure 6.

Subpopulation distribution dynamics following glucose perturbation for E. coli cells as a consequence of exposure to freeze-thaw treatment. Percentages of low-fluorescence (circles, black) and high-fluorescence (squares, gray) subpopulations vs. time following a 0.45 g.L⁻¹ glucose pulse for E. coli in aerobic, glucose-limited chemostats cultures frozen after growth at D = 0.1 h⁻¹ (A), D = 0.36 h⁻¹ (B), and D = 0.51 h⁻¹ (C), measured at pH 5.5. The black dotted line indicates the time of the glucose pulse. Full vertical lines correspond to the time when pulsed glucose was consumed under the indicated conditions. Vertical error bars indicate biological triplicates, however errors were mostly insignificantly small.