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. Author manuscript; available in PMC: 2020 Jul 9.
Published in final edited form as: Biochemistry. 2019 Jun 27;58(27):3042–3056. doi: 10.1021/acs.biochem.9b00225

Figure 1. The FF-Motif Extends Upon Calcium Binding and Does not Alter PAD2 Activity.

Figure 1.

(A) Surface (upper) and stick-cartoon (below) representations of apoPAD2 (PDB: 4N2O), 10 mM Ca2+-PAD2 (PDB: 4N2B) and holoFFA (PDB: 4N2C).12 (B) Heat map shows highly conserved (red) and the hyper-variable region (yellow) in PADs that encompasses the FF motif (boxed) in PAD2. (C) Catalytic efficiency and calcium dependence of PAD2, D177A, FFA, Δ221–222 and Δ220–223. Data are represented as mean ± SEM.