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. Author manuscript; available in PMC: 2020 Jul 9.
Published in final edited form as: Biochemistry. 2019 Jun 27;58(27):3042–3056. doi: 10.1021/acs.biochem.9b00225

Figure 4. PAD2 interacting proteins are modulated by calcium and PAD2 physically interacted with ANXA5.

Figure 4.

(A) Subcellular localization of biotinylated proteins associated with PAD2 with or without ionomycin stimulation. (B) Ratio of ratio generated from Figure 3C left two graphs. (C) BioID2 and BioID2-PAD2 transfected HEK-293T cells were treated with biotin with or without ionomycin. Biotinylated proteins were isolated using streptavidin-agarose and detected by anti-PAD2 and anti-ANXA5. (D) Recombinant PAD2 and ANXA5 were incubated at 37 °C for 2 h. anti-PAD2 was used to pull down ANXA5. Normalized density of precipitated ANXA5 is on the left (n = 3). (E) Immunoprecipitation of PAD2 transfected HEK-293T cells treated with or without ionomycin by anti-ANXA5 and detected with an anti-PAD2 antibody. Normalized density of precipitated ANXA5 is on the left (n = 2).