Skip to main content
. Author manuscript; available in PMC: 2020 Jul 9.
Published in final edited form as: Biochemistry. 2019 Jun 27;58(27):3042–3056. doi: 10.1021/acs.biochem.9b00225

Figure 5. PAD2 physically interacted with Ran.

Figure 5.

(A) BioID2 and BioID2-PAD2 transfected HEK-293 cells were treated with biotin with or without ionomycin. Biotinylated proteins were isolated on streptavidin agarose and proteins detected by western blotting using anti-PAD2 and anti-Ran antibodies. (B) IP was performed in PAD2 transfected HEK-293 cells treated with or without ionomycin by mouse anti-PAD2 and detected by rabbit anti-Ran and anti-PAD2. Normalized density of precipitated RAN is on the left (n = 2). (C) Recombinant PAD2 and Ran, RanQ69L, RanT24N were incubated at 37 °C for 2 h. anti-PAD2 was used to pull down Ran. Normalized density of precipitated RAN is on the left (n = 2).