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Aurigene compounds (1–3) show no inhibition between 30 nM and 30 μM for PD-1 flowing over PD-L1. Soluble PD-1 was flowed across surface-bound PD-L1 with and without test compounds at varying concentrations. The response is normalized to the control protein interaction (PD-1 only). Responses were measured in triplicate, and error bars represent standard deviation.
