Table 3. Tyrosyl Radicals in LmChdCa.
tyrosine residue | accessible surface area (Å2) | % modified (+46 Da) |
---|---|---|
Y61 | 0.1057 | 0.12 |
Y97 | 8.4632 | 0.00 |
Y102 | 19.4741 | 0.00 |
Y104 | 0.3495 | 0.00 |
Y113 | 1.3375 | 0.00 |
Y125 | 23.9246 | 0.00 |
Y135 | 16.1367 | 15.60 |
Y147 | 0.0000 | 56.04 |
Y160 | 1.4756 | 0.30 |
Y181 | 5.3286 | 0.80 |
Y198 | 16.2791 | n.d.b |
Y228 | 0.6343 | 0.00 |
A 30 μM portion of LmChdC, preincubated with excess MNP, was treated with 2.5 mM H2O2, and covalent modifications were identified using mass spectrometry.
Not detected.