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. 2019 Jul 9;9(18):5166–5182. doi: 10.7150/thno.33972

Figure 4.

Figure 4

HMGB1 activated Akt signaling pathway and increased key cell-cycle regulatory proteins. (A) Western blotting revealed that HMGB1 knockdown in PC-3 and LNCaP cells caused a decrease in p-Akt Ser473, CDK4, c-myc, cyclin D1, and cyclin E, along with an increase in p27, p21. (B) Expression of these proteins showed an opposite effect in 22Rv1 cells with ectopic-HMGB1. (C) Introduction of Akt into PC-3 and LNCaP HMGB1-shRNA cells restored the levels of CDK4, c-myc, cyclin D1, and cyclin E together with decreased expression of p21; the p27 level showed less change. (D) 22Rv1/Lv-HMGB1 cells were treated with Akt inhibitor LY294002 (50 μM) for 24 h. Inhibition of the Akt signaling suppressed the promoting effect of HMGB1 overexpression on the Akt pathway.