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. 2019 Jul 9;9(18):5122–5133. doi: 10.7150/thno.35773

Figure 5.

Figure 5

Bmal1 ablation sensitizes mice to hyperbilirubinemia (A) Schematic diagram for the experimental protocol of bilirubin-induced hyperbilirubinemia in WT and Bmal1-/-mice. (B) Measurements of UCB in plasma and livers of bilirubin or vehicle treated mice at ZT2 or ZT14. Data are mean ± SD (n=7). *P < 0.05 (post hoc Bonferroni test). (C) Measurements of CB in gallbladder of bilirubin or vehicle treated mice at ZT2 or ZT14. Data are mean ± SD (n=7). *P < 0.05 (t test). (D) Measurements of CB in plasma of bilirubin or vehicle treated mice at ZT2 or ZT14. Data are mean ± SD (n=7). (E) Schematic diagram for the experimental protocol of phenylhydrazine-induced hyperbilirubinemia in WT and Bmal1-/-mice. (F) Measurements of UCB in plasma and livers of phenylhydrazine-induced hyperbilirubinemia mice. (G) Measurements of ALT and AST in plasma of phenylhydrazine-induced hyperbilirubinemia mice. (H) Measurements of IL-1β, IL-6 and Tnfα mRNA levels in livers of phenylhydrazine-induced hyperbilirubinemia mice. (I) Representative histopathological image of livers from hyperbilirubinemia mice induced by phenylhydrazine. (J) Measurements of CB in gallbladder of phenylhydrazine-induced hyperbilirubinemia mice. (K) Measurements of CB in plasma and livers of phenylhydrazine-induced hyperbilirubinemia mice. In panel F, G, H, J&K, data are mean ± SD (n=6). *P < 0.05 (t test). Ve: vehicle; Bi: bilirubin.