In vivo toxicity of Zapadcine-1. MTD assay was conducted in male SD rats and cynomolgus monkey. The rats were single-dosed intravenously on days 1 with vehicle, 10 mg/kg, 20 mg/kg, 30 mg/kg or 40 mg/kg of Zapadcine-1 (n = 4). Body weight of SD rats (A) and survival rate (B) were observed. Kaplan-Meier survival plots show the percentage of rat survival over day 21. The cynomolgus monkeys were single-dosed intravenously on day 1 with vehicle, 2.0 mg/kg, 3.0 mg/kg, 4.0 mg/kg or 5.0 mg/kg of Zapadcine-1 (n = 1). (C) Body weight of cynomolgus monkey. Kaplan-Meier survival plots (D) show the percentage of animal survival over day 21.