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. Author manuscript; available in PMC: 2019 Aug 13.
Published in final edited form as: Biol Reprod. 2018 Feb 1;98(2):239–249. doi: 10.1093/biolre/iox177

Figure 6.

Figure 6.

Comparison of the dynamic range of testosterone production between the H295R steroidogenesis assay (Angela Buckalew, personal communication), the 24-well format of the Leydig cell (LC) assay, and the 96-well format of the Leydig cell assay. Markers represent mean ± SEM testosterone production (ng/106 cells) without (empty markers) and with stimulation (filled markers), respectively (10 μM forskolin = H295R assay; 100 ng/mL LH = LC assay) for two to eight independent replicates. Significant differences (P < 0.05) between unstimulated and stimulated testosterone production and differences between groups are denoted by different letters.