Figure 7.

Notch inhibitor effect on ZIKV infectivity, cytotoxicity and cell differentiation in D0-hNPCs. Cell lysates of mock and ZIKV-infected D0-hNPCs treated with DAPT or untreated were analyzed for cleaved Notch1, C-myc and Hes1 (A). Distribution of absolute counts of neurons, astrocytes, hNPCs and total cells was determined within 10 independent squares of 0.2 mm², using box plot (C). Comparison between DAPT treated and untreated cells was performed with a Tuckey’s range test with a P-value significant when *p < 0.05, **p < 0.01, ***p < 0.001. The percentage of infected cells were manually determined based on virus markers (C). Comparison between DAPT treated and untreated cells was performed with a Mann–Whitney test with a P-value significant when *p < 0.05, **p < 0.01, ***p < 0.001.