Table 3.

Effect of metal ions on activity of purified ligninolytic enzymes produced by Trametes polyzona KU-RNW027.

		Relative activity (%)
Reagent	Final concentration	MnP1	MnP2	Lac1
Metal ion (mM)
Na+	1	101.3 ± 0.2	120.5 ± 0.7	95.4 ± 1.1
	5	102.9 ± 0.5	127.5 ± 1.0	86.8 ± 0.3
	10	108.3 ± 1.2	143.0 ± 2.3	65.5 ± 0.5
K+	1	96.2 ± 0.3	149.5 ± 1.5	99.0 ± 0.6
	5	91.8 ± 0.6	135.5 ± 2.0	95.0 ± 0.1
	10	90.1 ± 0.5	124.0 ± 0.5	89.5 ± 0.2
NH4+	1	131.0 ± 0.2	93.00 ± 0.9	100.4 ± 0.3
	5	136.5 ± 0.7	92.7 ± 0.3	100.0 ± 0.5
	10	133.0 ± 0.5	89.5 ± 0.7	100.8 ± 1.0
Ca2+	1	138.0 ± 0.4	95.5 ± 1.1	93.3 ± 0.6
	5	134.5 ± 0.7	95.2 ± 0.3	89.4 ± 1.0
	10	126.5 ± 1.0	91.1 ± 0.5	82.3 ± 0.3
Mg2+	1	140.0 ± 0.5	97.1 ± 0.5	99.2 ± 0.7
	5	141.5 ± 1.4	97.1 ± 0.8	92.9 ± 0.9
	10	137 ± 0.3	94.6 ± 0.9	91.7 ± 1.2
Cu2+	1	146.5 ± 0.2	102.2 ± 0.7	100.4 ± 0.2
	5	135.0 ± 0.5	93.3 ± 0.4	100.8 ± 0.6
	10	90.5 ± 0.1	77.4 ± 0.5	100.0 ± 0.5
Mn2+	1	151.5 ± 0.5	105.7 ± 0.7	99.6 ± 0.6
	5	160.5 ± 0.9	108.9 ± 0.4	102.7 ± 0.9
	10	159.5 ± 0.2	115.0 ± 0.5	109.5 ± 1.1
Fe2+	0.01	141.0 ± 0.3	103.5 ± 1.2	99.6 ± 0.5
	0.02	ND	ND	65.8 ± 0.3
	0.03	ND	ND	12.2 ± 0.5
	0.04	ND	ND	2.0 ± 0.2
	0.05	151.0 ± 0.5	96.5 ± 0.2	0.0 ± 0.0
	0.1	132.5 ± 0.7	70.7 ± 0.5	0.0 ± 0.0
	0.5	53.0 ± 1.1	33.4 ± 0.2	0.0 ± 0.0
	1	0.0 ± 0.1	2.2 ± 0.3	0.0 ± 0.0
	5	ND	0 ± 0.0	0.0 ± 0.0
	10	ND	ND	ND
Co2+	0.01	ND	ND	99.2 ± 0.7
	0.05	ND	ND	106.3 ± 0.8
	0.1	ND	ND	103.1 ± 0.5
	0.5	ND	ND	102.4 ± 1.1
	0.75	ND	ND	39.8 ± 0.9
	1	153.5 ± 0.7	100.3 ± 0.8	0.0 ± 0.0
	5	139.0 ± 1.5	100.0 ± 0.5	ND
	10	108.0 ± 0.2	99.4 ± 0.7	ND
Zn2+	1	132.0 ± 0.3	100.6 ± 0.4	100.4 ± 0.8
	5	120.5 ± 0.5	89.5 ± 0.5	109.1 ± 1.0
	10	114.0 ± 0.3	85.7 ± 0.2	99.6 ± 0.3
Ag+	1	161.5 ± 1.2	107.6 ± 0.8	172.1 ± 0.5
	5	134.0 ± 0.5	83.8 ± 0.6	134.6 ± 1.3
	10	101.0 ± 0.7	60.2 ± 0.5	119.3 ± 0.7
Hg2+	1	100.5 ± 0.5	117.8 ± 0.4	100.4 ± 0.5
	5	130.5 ± 1.0	115.0 ± 0.5	59.5 ± 0.1
	10	211.0 ± 0.9	102.2 ± 1.5	28.4 ± 0.1

ND: not determined. Activation of the enzyme activities were indicated in bold.