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. 2019 Aug 6;10:1788. doi: 10.3389/fimmu.2019.01788

Figure 2.

Figure 2

The −376/−295 bp region of the mouse IL-10 promoter mediates the early synergistic effect of cAMP. RAW264.7 macrophages, transfected with either of the indicated mouse IL-10 promoter deletion mutant reporter constructs, were incubated with LPS (10 ng/ml) and isoproterenol (Iso, 1 μM) for 3 or 24 h. Luciferase reporter data represent three independent experiments and are expressed as mean ± SD of values normalized against renilla luciferase activity, divided by unstimulated control cells and relative (in %) to full (1,538 bp) promoter reporter activity (for which stimulation by LPS+Iso was 35.5-fold and 40-fold, relative to resting cells, at 3 h and 24 h, respectively); ***p < 0.001, ****p < 0.0001 for cells transfected with the indicated deletion mutants compared to cells transfected with the −295 bp (3 h) or −78 bp (24 h) promoter reporter (two-way ANOVA followed by Dunnett's post-test). The experiment was carried out 3 times.