Table 1.

K_d values for binding of CREB and AP-1 heterodimers to CRE sequences.

Oligo	Positiona		Sequence		Kd (μM) for various TF dimers
	Site	Context			CREB/ATF1	CREB	ATF1	c-Jun/c-Fos	c-Jun/ATF2
CREb	–	−349/−315	consensus	TGACGTCA	0.06	0.07	0.03	0.15	0.05
CRE1	−357/−350	−368/−336	wt	CCACGTCA	0.06	0.56	0.05	3	2
			mutant	CCAaaaat	84	47	32
CRE2	−335/−329	−349/−315	wt	TG-CGTGA	13	66	16	n.d.c	n.d.c
			mutant	Ta-aaTGA	n.d.c	109	48
CRE1+ CRE2	As above	−366/−298	wt	As above	0.05	1.6	0.08
			mut CRE1	As above	10	61	10
TREb	–	−349/−315	consensus	TGA-GTCA	12	53	17	0.5	9

^aPosition in the mouse IL-10 promoter, relative to TSS. Site = cis element, context = promoter region present in the oligonucleotide used for the binding assay.

^bThe depicted consensus sequence (40) was inserted in the context of the IL-10 promoter −349/−315 oligonucleotide, replacing the CRE2 sequence. Similar results were obtained when it was inserted in the context of the TNFα promoter CRE region.

^cn.d., no binding detected.