Figure 3.

Three significant SNPs were validated for population genetics using direct sequencing in Chinese Holstein cows. (A) Gel electrophoresis pattern PCR amplified fragments near three significant SNPs, a-c were PCR amplified fragments of SNPs rs88640083, rs75762330, and rs20438858 regions, respectively. (B–D) Directing sequencing results of PCR amplification products near above three important SNPs, and their alignment with reference sequences (ref: reference sequences; 1: heterozygous sequences; 2: variant sequences). The purple boxes were where the three SNPs located. X, M, and N represented the heterozygous types of the three SNPs, respectively.