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Hydrophobic change of PEG-Hb at acid pH values. (A) Changes in Trp fluorescence in Hb solutions with pH values of 6.6, 6.0, and 4.0. (B) Exposure of hydrophobic domains of Hb at pH 6.6, pH 6.0, and pH 4.0 as assessed by fluorescence emission spectra of ANS bound to PEG-Hb.
