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. 2019 Aug 2;13:769. doi: 10.3389/fnins.2019.00769

TABLE 1.

Condition of real-time PCR.

PCR condition
Stage Temperature (C) Time (min:s)
Initial denaturation 95 3:00
Denaturation 95 ↓× 39 cycle 0:10
Annealing 55 0:30
Initial extension 95 0:10
Final extension 65 0:05