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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Mol Endocrinol. 2019 Aug 1;63(2):123–138. doi: 10.1530/JME-19-0021

Figure 5. Overexpression of YTHDF proteins enhances protein levels for several genes found to be upregulated in slow aging mice.

Figure 5.

A) Representative western blot of target protein levels in HEK cells transfected with control vector, or with YTHDF1 or YTHDF2 vectors. B) Bars represent the mean ± S.E.M.SEM from 8 independent transfections. All values were normalized to 1 for the respective controls. C) CT values of qRT-PCR performed using mRNA purified from at least 8 independent transfections per group. The (*) indicates statistical significance (p<0.05) relative to controls. D) The bar represents the mean ± S.E.M. from at least 8 independent transfections, showing treatment with or without rapamycin (Rapa+ or Rapa-) in enhancing the effects of YTHDF on protein expression. (*) indicates statistical significance (p<0.05) relative to rapamycin untreated cells (Rapa -) from same group.