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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: J Mol Endocrinol. 2019 Aug 1;63(2):123–138. doi: 10.1530/JME-19-0021

Figure 8. 4EGI-1 upregulates protein degradation and enhances stress resistance in mouse fibroblasts.

Figure 8.

A) Representative western blots comparing protein degradation rates of MGMT, NDRG1, TFAM, LONP1, YTHDF1 and PGC1 in UM-HET3 fibroblasts, comparing untreated (controls) or 4EGI-1 treated cells at different time points during cycloheximide treatments. B) Mean ± S.E.M. of the half-life relative to actin for each protein from at least 3 independent fibroblast lines for each genotype of mice. Each symbol represents cells from a different mouse, while (*) indicates statistical significance (p<0.05) compared to controls. C) Effects of 4EGI-1 on stress resistance: LD50 (50% lethal dose) values for MMS and tunicamycin treatments in at least 4 experiments from at least 4 different mouse fibroblast donors. The (*) indicates statistical significance (p<0.05) relative to controls.