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. 2019 Aug 9;12:6297–6307. doi: 10.2147/OTT.S214689

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© 2019 Sun et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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MALAT1 promoted cell proliferation and inhibited cell apoptosis in SKOV3 and OVCAR3 cells.

Notes: The cell growths of SKOV3 (A) and OVCAR3 (B) cells were monitored using MTT assays in response to si-MALAT1 or pcDNA3.1-MALAT1 induced MALAT1 knockdown or overexpression, respectively. Effects of MALAT1 knockdown or overexpression on cell proliferations of SKOV3 (C) and OVCAR3 (D) were further determined by Edu staining (200×). (E) Apoptosis of SKOV3 and OVCAR3 cells transfected with si-MALAT1, si-NC, pcDNA3.1-MALAT1, or pcDNA3.1 was analyzed by flow cytometry. The percentage displayed on the histograms indicates apoptotic rate. Comparison of apoptotic rates of cells transfected with si-MALAT1, si-NC, pcDNA3.1-MALAT1, or pcDNA3.1. (F) Proteins were detected by Western blot. β-Actin was used as internal control to ensure equal loadings. Graphs are represented as the mean density of p53, Bax, Bcl-2, and Survivin bands normalized against the mean density of β-actin band from three independent experiments (presented as relative density of individual protein). Data are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01, ***P<0.001 vs si-NC, or pcDNA3.1.

Abbreviations: MALAT1, metastasis-associated lung adenocarcinoma transcript 1; si-MALAT1, MALAT1 small interfering RNA; si-NC, noncoding small interfering RNA.