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. 2019 Aug 9;12:6297–6307. doi: 10.2147/OTT.S214689

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© 2019 Sun et al.

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MALAT1 regulated OC cell proliferation and apoptosis through interaction with miR-503-5p.

Notes: (A and B) The cell growth was monitored in response to co-processing MALAT1 knockdown and miR-503-5p inhibition using MTT assay in both SKVO3 and OVCAR3 cells. (C and D), Edu staining was applied to observe the co-effect of MALAT1 knockdown and miR-503-5p inhibition on cell proliferation on SKVO3 and OVCAR3 cells (200×). (E) Apoptosis of SKOV3 and OVCAR3 cells co-transfected si-MALAT1/si-NC and miR-503-5p inhibitor/inhibitor NC was analyzed by flow cytometry. The percentage displayed on the histograms indicates apoptotic rate. Comparison of the apoptotic rates of cells co-transfected with si-MALAT1/si-NC and miR-503-5p inhibitor/inhibitor NC. (F) Apoptotic or antiapoptotic proteins were detected by Western blot. β-Actin was used as internal control to ensure equal loadings. Graphs are represented as the mean density of p53, Bax, Bcl-2, and Survivin bands normalized against the mean density of β-actin band from three independent experiments (presented as relative density of individual protein). Data are presented as the mean ± standard error of the mean (n=3). *P<0.05, **P<0.01 vs si-NC or blank control.

Abbreviations: MALAT1, metastasis-associated lung adenocarcinoma transcript 1; si-MALAT1, MALAT1 small interfering RNA; si-NC, noncoding small interfering RNA.