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. Author manuscript; available in PMC: 2020 Aug 1.
Published in final edited form as: Epilepsy Res. 2019 Apr 15;154:8–12. doi: 10.1016/j.eplepsyres.2019.04.006

Figure 2. The effect of stiripentol on spike-wave discharges in Gabrg2+/Q390X knockin mice.

Figure 2.

(A, B) Representative EEG recordings showing spike-wave discharges (SWDs) in a 3-month old Gabrg2+/Q390X knockin mouse treated with stiripentol alone (STP,150mg/kg, ip) (A) or with stiripentol plus diazepam (DZP,0.3mg/kg, ip) (B). In A and B, boxed regions in the left panel were expanded in the right panel. (C) The occurrence of the SWDs in the wildtype (wt) or Gabrg2+/Q390X heterozygous (het) mice was measured over a 24-hr session for baseline recording without any treatment or with stiripentol (N=15 for wt control, N=8 for wt STP; N=18 for het control and 8 for het STP, ***P<0.001 vs wt in control, §§§ vs het in control, genotype and treatment were taken as independent variables. P<0.001 for interaction F (1,43)=39.93; genotype F(1,43)=136.8 and for treatment F(1,43)=50.22, two-way ANOVA Bonferroni posttests). The first 10 min recordings of each hour were evaluated. (D). The total number of SWDs in the wt or het mice was measured over a 3-hr recording session. The first 15 min of each hour of recordings after drug administration (15 min after administration) was evaluated. Mice were treated with 0.9% normal saline (Vehicle, Veh), stiripentol (150mg/kg, STP, ip), Diazepam (0.3mg/kg, DZP, ip) alone or a combination of STP and DZP ((for wt, N=8 for vehicle and STP, 10 for DZP and 7 for STP+DZP, for het, N=11 for all four conditions. P<0.0001 for interaction (F(3,69)=22.49; drug treatment (F(3,69)=26.33 and genotype (F(1,69)=73.40)). (E). Stirpentol alone increased the SWDs in EEG in Gabrg2+/Q390X knockin mice but DZP alone or in combination with stiripentol reduced the SWDs after a pentylenetetrazol (PTZ, 50mg/kg, ip) seizure induction (for wt, N=9 for vehicle, STP and DZP respectively and N=6 for STP+DZP; for het, N=8 for all four conditions, P<0.0001 for interaction (F(3,57)=33.53; for drug treatment (F(3,57)=49.53 and for genotype) (F(1,57)=77.76)). In D and E, *P<0.05, ***P<0.001 vs wt with the same treated condition; §P<0.05, §§§ P<0.001 vs het vehicle. In C, D, E, drug treatment and genotype are the independent variables.