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. 2019 May 30;14(9):850–876. doi: 10.1080/15592294.2019.1615357

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 8. — Detection of 3ʹUTR sequences of YAP1 transcripts in bovine muscle. (a) Design of primer sets for YAP1 transcript XM_015474584.1 and XM_015474589.1. Black arrows = YAP1 gene. Green rectangles = exons. Purple lines = coding sequences. Yellow lines = 3ʹUTR sequences. Blue/red/brown/black lines = amplicon sequences of different primer sets. Numbers indicate expected length of each sequence. (b) PCR products of primer set3 and set4 with genomic DNA (gDNA), cDNA, and water (-/-) as templates on 0.5% agarose gel. (c) PCR products of primer set1 and set2 with cDNA and water (-) as templates on 1% agarose gel. (d) Sanger sequencing results of the two bands detected in (c). Locations of the primers are indicated on the top. F = forward primer. R = reverse primer.