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qRT-PCR determination of IGF2 expression in human tongue. The level of IGF2 was normalized to the mean of three endogenous control genes (GAPDH, ACTB, and ATP5PB). Mann-Whitney U Test was performed for qRT-PCR results and significant difference between hControl and BWS groups was indicated by p value. Unfilled bars = mean and standard deviation of hControl tongue samples. Filled bars = mean and standard deviation of BWS tongue samples.
