Table 1. List of primary antibodies used to characterize normal adult sural nerves.
The primary antibody list to detect selected proteins in normal peripheral nerves based on data obtained from the human BNB transcriptome includes detailed information of the antibodies, including final concentrations used for indirect fluorescent immunohistochemistry.
| Gene Symbol |
Protein Name | Antibody Source |
Catalog Number |
Host/Isotype, Stock Concentration |
Final Concentration |
|---|---|---|---|---|---|
| ABCB1 | P-glycoprotein | Life Technologies | MA5-13854 | Mouse / IgG1, 0.2 mg/mL | 4 μg/mL |
| ACTG1 | Gamma-1 actin | Life Technologies | PA5-13467 | Rabbit / IgG, 2 mg/mL | 40 μg/mL |
| AQP1 | Aquxaporin 1 | Santa Cruz Biotechnology | sc-25287 | Mouse/ IgG1 (kappa light chain), 200 μg/mL | 4 μg/mL |
| CALD1 | Caldesmon 1 | Abcam | ab68878 | Rabbit/ Polyclonal IgG, 0.3 mg/mL | 1 μg/mL |
| CAV1 | Caveolin 1 | Santa Cruz Biotechnology | sc-53564 | Mouse/ IgG2b (kappa light chain), 200 μg/mL | 4 μg/mL |
| CD44 | Cell surface adhesion glycoprotein | Santa Cruz Biotechnology | sc-7297 | Mouse/ IgG1 (kappa light chain), 200 μg/mL | 4 μg/mL |
| CD63 | Tetraspanin family member | Santa Cruz Biotechnology | sc-5275 | Mouse/ IgG2a (kappa light chain), 200 μg/mL | 4 μg/mL |
| CDH5 | Cadherin 5 | Santa Cruz Biotechnology | sc-9989 | Mouse/ IgG1 (kappa light chain), 200 μg/mL | 4 μg/mL |
| CDH6 | Cadherin 6 | Life Technologies | MA1-06305 | Mouse / IgG1, 1 mg/mL | 20 μg/mL |
| CLDN4 | Claudin 4 | Life Technologies | 36-4800 | Rabbit / IgG, 0.25 mg/mL | 2.5 μg/mL |
| CLDN5 | Claudin 5 | Life Technologies | 35-2500 | Mouse / IgG1, 0.5mg/mL | 10 μg/mL |
| CTNNA1 | Alpha-1 catenin | Life Technologies | 13-9700 | Mouse / IgG1 kappa, 0.5 mg/mL | 2 μg/mL |
| ESAM | Endothelial cell adhesion molecule | R&D Systems | MAB4204 | Mouse/ IgG2b, 0.5 mg/mL | 10 μg/mL |
| FLNA | Filamin A | Santa Cruz Biotechnology | sc-17749 | Mouse/ IgG2a (kappa light chain), 200 μg/mL | 4 μg/mL |
| GJA1 | Gap junction protein alpha 1 | Life Technologies | 13-8300 | Mouse / IgG1, 0.5 mg/mL | 10 μg/mL |
| LIN7A | Lin 7A, crumbs cell polarity complex | Life Technologies | PA5-30871 | Rabbit / IgG, 1 mg/mL | 20 μg/mL |
| MPDZ | Multiple PDZ domain crumbs cell polarity complex component | Sigma-Aldrich | HPA020255 | Rabbit/ Polyclonal IgG, 0.1 mg/mL | 2 μg/mL |
| MYO10 | Myosin X | Life Technologies | PA5-55019 | Rabbit / IgG, 0.3 mg/mL | 6 μg/mL |
| PCDH1 | Protocadherin 1 | Life Technologies | PA5-35091 | Rabbit / IgG, 0.47 mg/mL | 9.4 μg/mL |
| SLC1A1 | Glutamate transporter | Cell Signaling Technology | 14501 | Rabbit/IgG, 160 mg/mL | 320 μg/mL |
| SLC16A1 | Monocarboxylate transporter 1 | Santa Cruz Biotechnology | sc-365501 | Mouse/ IgG1 (kappa light chain), 200 μg/mL | 4 μg/mL |
| SLC19A2 | Thiamine transporter | Life Technologies | PA5-53456 | Rabbit / IgG, 0.1 mg/mL | 2 μg/mL |
| TJP1 | Zona occludens-1 | Life Technologies | 61-7300 | Rabbit / IgG, 0.25 mg/mL | 2.5 μg/mL |
| VEZT | Vezatin | Life Technologies | PA5-52115 | Rabbit / IgG, 100 μL, 0.3 mg/mL | 6 μg/mL |
| ZYX | Zyxin | Life Technologies | PA1-25162 | Rabbit / IgG, 13.3 mg/mL | 133 μg/mL |