Appendix 1—figure 15. Simulation estimation chart of 1000 fluorophores.

The $y$-axis is the normalization of fluorophores in the $S_0$ state (emission) in the time $i$ ($x$-axis). The normalized data extracted from the images are shown in black. The purple and the brown dots represent the simulation of 1000 fluorophores as a Markov chain with the transition matrix that we propose and with the original 3B matrix, respectively. The transition matrix that we propose is shown in the bottom of the figure.