FIG 4.

siRNA screening of mouse CTA genes using the second cell lines. Changes of viability of liver (A), colon (B), bladder (C), ovary (D), lung (E), melanoma (F), and breast (G) cancer cell lines were tested using two different siRNAs (siRNA#1 and siRNA#22) corresponding to the CTA genes selected by using the first cell lines. KD was carried out in the second cell lines, i.e., lines that showed the next highest level of expression of the tested genes. Cell viability was determined by MTS assay at 72 h after transfection with the siRNAs. Viability of KD cells relative to that of control cells is shown. Graphs represent data from two biological replicates. Dots indicate values from each of the two replicates. Solid red lines show 100% viability; broken red lines indicate 10% increased or decreased viability. Red text indicates genes for which KD (by at least one siRNA) caused a viability change of >10%.