FIG 7.

Effect of tubastatin A (TBSA), a specific inhibitor of HDAC6, in OV3121 cells. (A) Disruption of β-III-tubulin (red) by Tekt5-KD in control cells (+DMSO) was rescued by TBSA. DAPI staining (blue) is also shown. siCTL, AllStars negative control siRNA. Scale bars, 50 μm. (B) The cell viability of Tekt5-KD OV3121 cells, but not that of control OV3121 cells, at 72 h posttransfection was increased by TBSA exposure. Cell viability was determined by an MTS assay. (C) Possible mechanism for TEKT5 control of cancer cell viability. PTM, posttranslational modification; Glu, glutamyl side chain; X, unknown factor(s) for polyglutamylation of tubulin. Values are plotted as means ± the SE. **, P < 0.01; ***, P < 0.001 (two-tailed, nonpaired Student t test).