FIG 7.
Repression of TSR4 blocks the nuclear export of pre-40S. The subcellular distribution of ITS1 was analyzed by FISH with a Cy3-labeled oligonucleotide complementary to the D-A2 region of ITS1 (tetramethyl rhodamine isothiocyanate [TRITC]) in xrn1Δ (RKY1977), PGAL1-RPS2 xrn1Δ (AJY4300), and PGAL1-3×HA-TSR4 xrn1Δ (AJY4297) cells after a switch to glucose-containing medium for 2 h. The position of the nucleus was determined by DAPI staining and the overall cell outline by differential interference contrast (DIC) microscopy.