Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Pseudomonas fluorescens) | SBW25; wild type | PMID: 8564013 | Ancestral strain | |
| Strain, strain background (Pseudomonas fluorescens) | SBW25-lacZ | PMID: 17669526 | SBW25 with neutral chromosomal lacZ insertion | |
| Strain, strain background (Escherichia coli) | DH5α λpir | PMID: 11207743 | E. coli cloning strain | |
| Recombinant DNA reagent | pAH79 (plasmid) | PMID: 24912567 | P. fluorescens allelic exchange vector | |
| Recombinant DNA reagent | gtsB mutagenesis vector (plasmid) | This paper | pAH79 modified for Golden Gate Assembly of mutant gtsB alleles | |
| Genetic reagent (Pseudomonas fluorescens) |
gtsB site-directed mutagenesis library | This paper | Collection of gtsB single nucleotide mutants in SBW25 background | |
| Recombinant DNA reagent | pUC18T-mini-Tn7T-Gm (plasmid) | PMID: 15908923 | GenBank: AY599232.2 | Source of mini-Tn7T-Gm transposon |
| Recombinant DNA reagent | pUC18T-mini-Tn7T-Gm-eYFP (plasmid) | PMID: 17406227 | GenBank: DQ493879.2 | Source of YFP |
| Genetic reagent (Pseudomonas fluorescens) | Mini-Tn7 gtsB-YFP transcriptional fusions | This paper | Transcriptional YFP fusions at SBW25 Tn7 site (see Figure 2A and B) | |
| Genetic reagent (Pseudomonas fluorescens) | Mini-Tn7 gtsB-YFP translational fusions | This paper | Translational YFP fusions at SBW25 Tn7 site (see Figure 2A) | |
| Genetic reagent (Pseudomonas fluorescens) | gts operon YFP transcriptional fusions | This paper | Transcriptional YFP fusions at sites within gts operon (see Figure 2C and Figure 2C—figure supplement 1) |