Table 1. Oligonucleotides used in this study.

Restriction enzyme recognition sequences are capitalised. BsaI overhangs are underlined. Introduced mutations are in bolded capital letters. Additional oligonucleotides used for site-directed mutagenesis are listed in Tables S1 and S2 of Supplementary file 1.

Name	Sequence (5’ to 3’)	Function
F2-pUC19-BsaI	gcgAGATCTgtcgtGAGACCggtgatgacggtgaaaacct	gtsB mutagenesis vector construction
R3-pUC19-MfeI-SpeI	actgcgACTAGTCAATTGattaatgcagctggcacgac	gtsB mutagenesis vector construction
F-800Right	actgcgCAATTGagaccccggaagacatcag	gtsB mutagenesis vector construction
R-800Right	actgcgTCTAGAcattgcgaagttcaagcgta	gtsB mutagenesis vector construction
F2-gtsB-F	actgcgGGTCTCagtcgaaaagtcgcgacctacatgg	Conserved gtsB forward primer
R3-gtsB-R	actgcgGGTCTCctgccggaCaccacggtcggccagctc	Conserved gtsB reverse primer
4845-M13F	GTAAAACGACGGCCAGTTCCGACAGGCTGTAGTCCTT	gtsB sequencing primer
R2-M13R-gtsB	GGAAACAGCTATGACCATGTGGTCCTCAGCTCGGAATA	gtsB sequencing primer
SP1	ACCACACCGAACAGGAAGTC	5’ RACE cDNA synthesis
B-SP2	ACTGCGTCTAGAGACCAAGGTGATACCGATAAACA	5’ RACE gtsB amplification
B-SP3	ACTGCGTCTAGACGAACAAGGCCAGGTTTTT	5’ RACE gtsB amplification
A-SP2	ACTGCGTCTAGATTTCTTGTCGAGCAGGGAGT	5’ RACE gtsA amplification
A-SP3	ACTGCGTCTAGATTCTTCTTTGGCGACGTCTT	5’ RACE gtsA amplification