Skip to main content
. Author manuscript; available in PMC: 2019 Aug 13.
Published in final edited form as: Nat Methods. 2019 Feb 25;16(3):225–234. doi: 10.1038/s41592-019-0330-1

Fig. 2 |. Subway map of CLIP protocols, from immunoprecipitation to PCR.

Fig. 2 |

The chart highlights steps of various representative CLIP-seq protocols. Not all steps are included. XL, UV cross-link; IP, immunopurification; phosphatase, removal of 3′ phosphate; kinase, addition of 5′ phosphate; RT, reverse transcription; L3, 3′ adaptor ligation to RNA or DNA; L5, 5′ adaptor ligation; PK extraction, proteinase K extraction from nitrocellulose membrane; Ppt/column, alcohol precipitation or column cleanup of nucleic acid; TBE, Tris-borate-EDTA; SA, streptavidin.