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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Mol Carcinog. 2019 May 16;58(9):1670–1679. doi: 10.1002/mc.23044

Figure 4. Flow cytometric analysis of IL-37 protein expression in immune cell subsets cultured with MCM.

Figure 4

(A) Gating strategy of human PBMCs for IL-37-expressing immune cell subsets. Following live cell gating, cell subsets were determined by antibodies against CD3, CD4, CD8, CD14, CD19, Foxp3, and CD56. Dot plots are representative of one of five donor PBMCs. (B) PBMCs were cultured in the absence (−) or presence (+) of 1205Lu-derived MCM (50%) for 24 h and subjected for antibody staining. Contour plots (left) and frequency of IL-37-expressing cells (right) in Tconv cells (gated CD4+Foxp3−), Treg cells (gated CD4+Foxp3+), CD8+ T cells, B cells (gated CD19+) and NK cells (gated CD56+), measured by flow cytometry. (C) Histogram (left) and quantification by mean fluorescent intensity (MFI) (right) of IL-37 expression in T cell subsets (Tconv cells in red, Treg cells in black, and CD8+T cells in blue) in the absence (−) or presence (+) of MCM. IgG control staining shown in gray. Data indicate mean ± SEM, n = 5. NS, not significant (p > 0.05); *, p < 0.05, **, p < 0.01, *** p < 0.001. Data are representative of two independent experiments.