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. 2019 Aug 13;9:11755. doi: 10.1038/s41598-019-47788-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Backward air trajectories of four example days (4/8 – 4/11/2015) prior to the infection confirmation date (4/12/2015) of the first Iowa case. Red, blue and green lines are the 24-h (12am-12am, Local Standard Time or LST), 16-h (12am-4pm, LST) and 8-h (12am-8am, LST) trajectories, respectively. The round red dot represents the first case location; other dots are cases (green for turkey, red for laying hen, purple for backyard) that had been confirmed positive before the first Iowa case and the infected flocks had not yet been depopulated.