Table 3.
Soil microbial community composition as impacted by long-term manure and fertilizer treatments for 0–7.5 cm depth at soybean planting (P).
| Treatments | G− | G+ | Anaerobe | Actinom | AMF | Fungi | Eukaryote | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| P | 1MAP | P | 1MAP | P | 1MAP | P | 1MAP | P | 1MAP | P | 1MAP | P | 1MAP | |
| ------------------------% ------------------------ | ||||||||||||||
| LM†† | 45.55ns | 47.91b | 15.38ns | 22.30ns | 1.79b | 2.85ns | 16.86ba | 11.86b | 3.21c | 3.36bc | 5.31ns | 4.18ns | 11.91ns | 6.44b |
| MM | 45.35 | 50.54b | 16.34 | 19.65 | 3.44a | 1.66 | 15.97bc | 12.54ba | 4.01ba | 5.00ba | 5.51 | 6.29 | 9.39 | 4.34b |
| HM | 45.73 | 51.36b | 17.44 | 19.13 | 3.12ba | 2.04 | 13.67c | 12.77ba | 4.15a | 5.72a | 4.93 | 5.41 | 10.97 | 3.59b |
| MF | 42.68 | 44.77b | 17.61 | 16.86 | 3.92a | 1.23 | 18.18ba | 16.25a | 3.39c | 3.41bac | 5.38 | 4.46 | 8.84 | 13.04a |
| HF | 41.32 | 48.63b | 23.08 | 18.98 | 3.89a | 1.52 | 18.31ba | 15.65ba | 3.49bc | 3.92bac | 2.72 | 4.92 | 7.20 | 6.38b |
| CK | 45.46 | 70.85a | 15.54 | 9.80 | 3.36a | 1.94 | 18.84a | 7.73c | 3.60bac | 1.70c | 5.31 | 2.89 | 7.89 | 5.09b |
| Analysis of Variance (P > F) | ||||||||||||||
| Treatment | 0.9 | 0.001 | 0.06 | 0.07 | 0.05 | 0.3 | 0.009 | 0.006 | 0.04 | 0.04 | 0.2 | 0.07 | 0.3 | 0.004 |
| M vs. F | 0.3 | 0.3 | 0.06 | 0.4 | 0.06 | 0.07 | 0.01 | 0.009 | 0.02 | 0.2 | 0.3 | 0.4 | 0.09 | 0.03 |
†Mean values followed by different lower letters between each treatment within each sampling time represent significant differences due to manure and inorganic fertilizer application at P < 0.05. ††LM, low manure rate based on recommended phosphorus rate; MM, medium manure rate based on recommended nitrogen rate; HM, high manure rate based on double of the recommended nitrogen rate; MF, recommended fertilizer; HF, high fertilizer; and CK, control with no manure application. ns, no significant.