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. 2019 Aug 13;9:11776. doi: 10.1038/s41598-019-48207-z

Table 4.

Soil PLFA community composition as impacted by long-term manure and fertilizer treatments for 0–7.5 cm depth at soybean planting (P) and one month after planting (1MAP).

Treatments G+/G− Bacteria Total Bacteria Total Fungi Bacteria/Fungi Ratio Total PLFA Biomass
P 1MAP P 1MAP P 1MAP P 1MAP P 1MAP
------------------------% mol------------------------ -nmol gr -1 soil-
LM†† 0.34ns† 0.47ns 79.58ns 84.91b 8.51ns 7.53b 9.42b 12.74b 79.91ns 87.07ns
MM 0.36 0.39 81.09 84.37b 9.53 11.29a 9.09b 7.48b 104.2 78.21
HM 0.38 0.37 79.96 85.29b 9.07 11.12a 8.81b 7.83b 117.9 94.75
MF 0.41 0.38 82.39 79.10c 8.77 7.87b 9.42b 10.34b 90.58 56.26
HF 0.56 0.39 86.6 84.79b 6.20 8.84ba 14.41a 9.74b 82.47 85.52
CK 0.34 0.14 83.21 90.32a 8.91 4.59c 10.17b 21.51a 27.15 57.53
Analysis of Variance (P > F)
Treatment 0.3 0.09 0.2 0.0001 0.2 0.001 0.04 0.0006 0.5 0.3
M vs. F 0.1 0.9 0.05 0.047 0.2 0.1 0.08 0.8 0.4 0.4

Mean values followed by different lower letters between each treatment within each sampling time represent significant differences due to manure and inorganic fertilizer application at P < 0.05. ††LM, low manure rate based on recommended phosphorus rate; MM, medium manure rate based on recommended nitrogen rate; HM, high manure rate based on double of the recommended nitrogen rate; MF, recommended fertilizer; HF, high fertilizer; and CK, control with no manure application. ns, no significant.