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. 2019 Aug 13;10(8):615. doi: 10.1038/s41419-019-1844-2

Fig. 7. MCT1 antagonizes the antitumor efficacy of OSI and is overexpressed in human CRC tissues.

Fig. 7

a Immunoblotting analysis of MCT1 protein levels in CRC cell lines and noncancerous colorectal cell line NCM460. b, c Representative images of colony formation assays of CRC cells transfected with siScramble or siMCT1 for 24 h, followed by treatment with or without 5 μM OSI for another 24 h. d Immunoblotting analysis of cleaved-PARP expression in CRC cells transfected with siScramble or siMCT1 for 24 h, followed by treatment with or without 5 μM OSI for another 24 h. e Immunoblotting analysis of MCT1 expression in 10 pairs of tumor and adjacent normal tissues obtained from CRC patients. f Relative quantification of MCT1 levels in (e). g Immunohistochemical staining of MCT1 in 10 pairs of tumor and adjacent normal tissues obtained from CRC patients. Scale bar, 50 μm. h Relative intensity of MCT1 levels in (g). i Data represent the Z-score of MCT1 from Oncomine database. Data are presented as mean SEM, Student’s t-test, and are representative of 3 independent experiments. **P < 0.01; ***P < 0.001