Table 2.
Comparison between RNA-Seq and QPCR.
| RNA-Seq | QPCR | |||
|---|---|---|---|---|
| Gene | Control Counts | Osteo Counts | Fold Change | Fold Change |
| Col10A1 | 5.00 | 281.00 | 56.20 | 0.98 ± 0.14 |
| Col11A1 | 545.58 | 10800.60 | 19.80 | 27.83 ± 11.71 |
| FBLN1 | 37245.64 | 130866.10 | 3.51 | 4.27 ± 0.67 |
| FERMT1 | 18.00 | 67.00 | 3.72 | 1.64 ± 0.35 |
| FN1 | 1222356.00 | 3391541.00 | 2.77 | 3.74 ± 1.17 |
| FOXF1 | 1.00 | 3.34 | 3.34 | 0.28 ± 0.03 |
| IGF1 | 50.92 | 137.92 | 2.71 | 2.94 ± 0.86 |
| LAMA3 | 93.00 | 425.97 | 4.58 | 1.17 ± 0.45 |
| LAMA4 | 14447.68 | 37124.57 | 2.57 | 4.00 ± 1.09 |
| LAMB1 | 8165.41 | 36493.15 | 4.47 | 4.62 ± 0.26 |
| WNT10B | 1.00 | 3.00 | 3.00 | 0.19 ± 0.05 |
| MMP1 | 2327.16 | 64.99 | 0.02 | 0.03 ± 0.01 |
| MMP3 | 2299.93 | 51 | 0.02 | 0.61 ± 0.26 |
| MMP16 | 599.47 | 48.02 | 0.08 | 0.22 ± 0.02 |
| ADAMTS6 | 764.97 | 184 | 0.24 | 0.56 ± 0.28 |
| ADAMTS14 | 833.36 | 45.01 | 0.05 | 1.46 ± 0.45 |
| ANGPT1 | 120 | 1639.72 | 13.66 | 14.83 ± 2.17 |
| HGF | 68.79 | 935.39 | 13.59 | 4.38 ± 0.44 |
| CCL2 | 1101.39 | 7375.72 | 6.69 | 0.61 ± 0.01 |
| CXCL1 | 32 | 121 | 3.78 | 29.16 ± 7.81 |
| CXCL5 | 79 | 376.09 | 4.76 | 10.83 ± 1.84 |
| CXCL6 | 59 | 136.87 | 2.31 | 3.46 ± 0.10 |
| CXCL8 | 38 | 87 | 2.28 | 18.83 ± 0.90 |