Fig. 5. Nonapoptotic cell death in GMR > egr/GMR-p35 shows morphological features of necrosis.

TEM images of wt (a), GMR > egr/ + (b, b′) and GMR > egr/GMR-p35 (c–c′′) disks at APF22h. b′ is an enlarged image for the outlined area in b. c′, c′′ are enlarged images for the outlined areas in c. Compared with the wild-type eye disk cell which has a large nucleus (yellow arrow, a), apoptotic features such as high-electron-density chromatin condensation (yellow arrows, b) and apoptotic bodies (dark aggregates in b and b′) are frequently observed in GMR > egr disks (b). Expression of P35 in GMR > egr (c), however, induces necrotic cell features such as translucent cytoplasm, mal-shaped (arrow, c′′) or unidentifiable nuclei (c′), and aggregation of endoplasmic reticulum and other cellular organelles (arrowheads, c′ and c′′). Asterisk indicates a phagolysosome