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. 2019 Aug 13;9:11793. doi: 10.1038/s41598-019-48141-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pri-miR-137 PCR Exhibits Multiple Banding Pattern. (a) PCR results are shown for pri-miR-137 custom primer pairs in adult and fetal total brain cDNA. Exon primer pairs are labeled along the top of each gel lane (i.e. E1-5 is primer pair exon 1 to exon 5). Ladders and corresponding base sizes are shown on either side. Results for both adult and fetal total brain tissues depict correct product lengths for all primer pairs (E1-5:2034, E2-5:1797, E3-5:1694, E4-5:1307, E5-5:838). A consistent multiple banding patterns can be seen for all reactions containing exon 3, indicating alternative splicing within exon 3. (b) A magnified view of PCR results for primer pair E3-5 demonstrates potential differential transcript expression between time points with brighter bands of expected size relative to shorter bands in the adult tissue and equal brightness between band sizes in the fetal tissue. (a) Images were cropped from separate gels. (b) Images cropped from the same gel. Uncropped gel images are presented in Supplemental Fig. S1.