Table 2.

Statistical assessment of 2-transcript Disease Risk Score (DRS) assay to differentiate between viral and bacterial groups using different reference gene(s) to normalize the expression.

	RG	p-values			AUC (%)
		Shapiro-Wilk test	f-test (variances)	t-test (groups)
Single RG normalization	GUSB	0.286	0.618	7.03 × 10−5	92.2
	PGK1	0.222	0.253	2.37 × 10−4	89.0
	TBP	0.288	0.927	8.62 × 10−5	93.5
	GAPDH	0.185	0.260	1.66 × 10−4	90.3
	ACTB	0.210	0.258	7.41 × 10−5	92.2
Multiple RG normalization	GUSB-PGK1	0.265	0.364	1.37 × 10−4	91.0
	GUSB-PGK1-TBP	0.296	0.495	1.04 × 10−4	92.2
	GUSB-PGK1-TBP-GAPDH	0.259	0.401	1.16 × 10−4	91.6

AUC: area under the ROC curve; RF: reference gene.