Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Aug 13;9:11791. doi: 10.1038/s41598-019-48071-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Total and membrane expression of wt NALCN channel and its L509S and Y578S variants. (A) Representative cell surface biotinylation experiment. The cell surface biotinylation assay was performed in cells transfected with NLF1 alone (Mock) or along with wt NALCN, NALCN-L509S or NALCN-Y578S (GFP tagged). The Na⁺/K⁺ ATPase, a plasma membrane protein, and the calreticulin, a reticulum-resident protein, were used internal controls. (B) The expression levels of wt NALCN, NALCN-L509S and NALCN-Y578S were normalized with the Na⁺/K⁺ ATPase level from both total and membrane fractions. The total expression compared to NALCN was: L509S, 62.99 ± 8.725%, p < 0.01; Y578S, 56.24 ± 9.408%, p < 0.01, while the membrane expression compared to NALCN was: L509S, 63.94 ± 7.261%, p < 0.01; Y578S, 46.69 ± 7.412%, p < 0.001 (n = 6 independent experiments). Statistical significance was calculated with a one-way ANOVA multiple comparisons test.