Figure 1.

Effects of serum from burned patients receiving standard of care or standard of care and pamidronate on C2C12 myotubes (N = 5 per group). Assessment of myofiber size in C2C12 myotube cultures exposed to 1% (A) or 5% (B) serum for up to 48 h obtained from 3 groups of 5 children: normal unburned (N), burned receiving standard of care after 30 d (B), burned receiving standard of care and pamidronate after 30 d (B+P) (n = 250–350 myofibers). Green staining: myosin heavy chain (MyHC). Data (means ± standard deviation) are expressed in micrometers (μm). Significance of the differences: ^*p < 0.05, ^**p < 0.01 vs. N, and ^#p < 0.01 vs. B. Significance was determined by two-way analysis of variance (ANOVA) followed by Tukey's post-test.