Figure 3.

Serum from burn patients receiving pamidronate normalized anabolic signaling compared to serum from burn patients (N = 4 per group). Representative Western blotting and quantification of pAKT, AKT, pmTOR, and mTOR in protein extract of murine C2C12 myotubes exposed for up to 48 h to 5% serum obtained from 3 groups of children: normal unburned (N), burned receiving standard of care after 30 d (B), and burned receiving standard of care and pamidronate after 30 d (B+P). Data were normalized to total protein. Data (means ± standard deviation) are expressed as arbitrary units (A.U.). Significance of the differences: ^*p < 0.05, ^**p < 0.01 vs. N and ^#p < 0.05, ^##p < 0.01 vs. B. Significance was determined by two-way analysis of variance (ANOVA) followed by Tukey's post-test.