Figure 3.

PI3K/Akt and IKK pathways were involved in IL-36β-mediated mTORC1 activation of CD8⁺ T cells. Naïve CD8⁺ T cells were isolated from C57BL/6j mice and stimulated with or without plate-bound 10 μg/ml anti-CD3 mAb, in the presence or absence of IL-36β (100 ng/ml) for various lengths of time. (A) Phosphorylation of Akt and S6 at different time points as indicated were determined by western blot. (B) Degradation of IκB at different time points as indicated was measured by western blot. (C,D) In the presence or absence of PI3K or IKK inhibitor, phosphorylation of S6 at 48 h was measured by flow cytometry (C) and western blot (D). Data are shown as mean ± SEM. *p < 0.05 and **p < 0.01 by unpaired t-test. The experiment was repeated independently three times.