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. 2019 Aug 7;10:1866. doi: 10.3389/fimmu.2019.01866

Figure 6.

Figure 6

C5aR1 deficiency reduced pDCs infiltration in psoriatic lesions. C5aR1+/+ and C5aR1−/− mice were treated with IMQ (Vaseline cream treatment as a control) on the shaved ear or back skin for indicated days, and then skin samples were obtained and infiltrated cells of skin lesions were isolated. (A) Frequencies of pDCs (PDCA-1+ cells) in the infiltrated cells of psoriatic ear and back skin lesions and (B) C5aR1 expression on these pDCs in C5aR1+/+ mice following 3 days IMQ application. (C) Frequencies of pDCs (PDCA-1+) in IMQ-treated back skin of C5aR1+/+ and C5aR1−/− mice were compared at indicated times. Percentages of PDCA-1+ pDCs is shown (n = 5–6). (D) Infiltration of pDCs (PDCA-1+) on day 3 in IMQ-treated back skin tissues of C5aR1+/+ and C5aR1−/− mice was measured by immunofluorescence. Arrows indicate positively stained cells. Scale bar = 5 μm. (E) PDCA-1 expression in IMQ-treated back skin tissues of C5aR1+/+ and C5aR1−/− mice was analyzed by qRT-PCR following 3 days IMQ treatment. (n = 5–6/group). Gating strategy is shown in Figure S5. Values are presented as mean ± SEM and data were obtained from three independent experiments. *p < 0.05; **p < 0.01.